Composite

Part:BBa_K190037:Design

Designed by: Michael Verhoeven   Group: iGEM09_Groningen   (2009-09-07)


GVP with pBad/araC promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1552
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 5357
    Illegal XhoI site found at 5045
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2003
    Illegal NgoMIV site found at 2405
    Illegal AgeI site found at 979
    Illegal AgeI site found at 5324
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961
    Illegal SapI.rc site found at 3815
    Illegal SapI.rc site found at 4971


Design Notes

Melbourne GVP cluster was used containg the 400bp repeat in gvpL


Source

E.coli and Bacillus

References